Anti-TNFAIP3 antibody [EPR2663] ab92324 from Abcam

Product Specs
ItemAnti-TNFAIP3 antibody [EPR2663]
CompanyAbcam
Price Supplier Page View Supplier Product Page
Catalog Numberab92324
Conjugate/TagUnconjugated
Quantity10 UL, 40 UL, 100 UL
ClonalityMonoclonal
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
CloneEPR2663
HostRabbit
IsotypeIgG
Antibody TypePrimary
Gene NameTNFAIP3
ValidationKnockOut Validated
ReactivityHuman, Mouse
TargetTNFAIP3

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(1) LncTUG1 ameliorates renal tubular fibrosis in experimental diabetic nephropathy through the miR-145-5p/dual-specificity phosphatase 6 axisRenal failureDecember 1, 2023Wang T, Cui S, Liu X, Han L, Duan X, Feng S, Zhang S, Li G.The membrane was blocked by 5% skimmed milk (BD, CA,USA) for 1h. Primary antibodies for TNFα (1:1000, ab92324, Abcam, Cambridge, MA, USA), IL-6 (1:1000, ab259341, Abcam) DUSP6 (1:2000, # 10433-1-AP, Proteintech Group, Inc

(2) Human osteoarthritic articular cartilage stem cells suppress osteoclasts and improve subchondral bone remodeling in experimental knee osteoarthritis partially by releasing TNFAIP3Stem Cell Research & TherapySeptember 27, 2023Zhi-Ling Li, Xiao-Tong Li, Rui-Cong Hao, Fei-Yan Wang, Yu-Xing Wang, Zhi-Dong Zhao, Pei-Lin Li, Bo-Feng Yin, Ning Mao, Li Ding, et al.transfer, PVDF membranes were incubated with anti-GAPDH (Proteintech, 10,494-1-AP) and anti-TNFAIP3 (Abcam, ab92324). ACSCs were cultured in the presence of TNF-α for 72 h and TNFAIP3 concentrations in the culture supernatants

(3) Degradation of Ubiquitin-Editing Enzyme A20 following Autophagy Activation Promotes RNF168 Nuclear Translocation and NF-κB Activation in Lupus NephritisJournal of Innate ImmunityMarch 21, 2023Luxi Zou, Ling Sun, Ruixue Hua, Yu Wu, Linlin Sun, Ting ChenHelsinki. Immunohistochemical Staining The primary rabbit antibodies to RNF168 (ab235892, 1:800), and A20 (ab92324, 1:80), along with the horseradish peroxidase (HRP)-labeled secondary antibody to immunoglobulin (IgG)

(4) A20 affects macrophage polarization through the NLRP3 inflammasome signaling pathway and promotes breast cancer progressionExperimental and Therapeutic MedicineFebruary 15, 2023Yanbin Zheng, Shenglan Wang, Yutong Zhong, Chunhong Huang, Xinjie Wunon-fat milk for 2 h at room temperature, and incubated with primary antibodies against A20 (cat. no. ab92324; dilution, 1:1,000; Abcam), VEGFA (cat. no. ab214424; dilution, 1:1,000; Abcam), NLRP3 (cat. no. ab263899;

(5) Chronic exposure to low-level lipopolysaccharide dampens influenza-mediated inflammatory response via A20 and PPAR networkFrontiers in ImmunologyJanuary 16, 2023Yinuo Gu, Alan Chen-Yu Hsu, Xu Zuo, Xiaoping Guo, Zhengjie Zhou, Shengyu Jiang, Zhuoer Ouyang, Fang Wangtemperature, and probed with specific primary antibodies (1:1000) against p65 (ab16502), p-p65 (ab76302), A20 (ab92324), NLRP3 (ab214185), pro-caspase-1 (ab179515), caspase-1 (ab179515), PPAR-α (ab61182), PPAR-γ (ab209350)

(6) Theaflavin-3,3’-Digallate Ameliorates Collagen-Induced Arthritis Through Regulation of Autophagy and Macrophage PolarizationJournal of Inflammation ResearchJanuary 10, 2023Lei Zhang, Wenming Li, Zhenyang Hou, Zhidong Wang, Wei Zhang, Xiaolong Liang, Zerui Wu, Tianhao Wang, Xin Liu, Xiaole Peng, et al.Life Technologies (USA, # 12571089, #10099141). Primary antibodies against F4/80 (#ab6640, 1:1000), TNF-α (#ab92324, 1:1000) and IL-1β (#ab205924, 1:1000) were obtained from Abcam (UK), and antibodies against iNOS (#13120,

(7) NF-κB/RelA controlled A20 limits TRAIL-induced apoptosis in pancreatic cancerCell Death & DiseaseJanuary 3, 2023Claudia Geismann, Charlotte Hauser, Frauke Grohmann, Christian Schneeweis, Nico Bölter, Jan-Paul Gundlach, Günter Schneider, Christoph Röcken, Christian Meinhardt, Heiner Schäfer, et al.examined by immunoblotting as described [13] with the use of primary antibodies: A20 (1:1000, Abcam Cat# ab92324, RRID:AB_10561788), PARP (1:1000, Cell Signaling Technology Cat# 9542, RRID:AB_2160739), Caspase 8 (1:1000,

(8) TRIM56 promotes malignant progression of glioblastoma by stabilizing cIAP1 proteinJournal of experimental & clinical cancer research : CRDecember 6, 2022Yang X, Zhang Y, Xue Z, Hu Y, Zhou W, Xue Z, Liu X, Liu G, Li W, Liu X, et al.cat#10,022–1-AP), cIAP1 monoclonal antibody (Proteintech, cat#66,626–1-Ig), TNFAIP3 monoclonal antibody (Abcam, cat#ab92324), recombinant anti-DDDDK tag antibody (Abcam, cat#ab205606), recombinant anti-ubiquitin (linkage-specific

(9) Fibroblast A20 governs fibrosis susceptibility and its repression by DREAM promotes fibrosis in multiple organsNature communicationsOctober 26, 2022Wang W, Bale S, Wei J, Yalavarthi B, Bhattacharyya D, Yan JJ, Abdala-Valencia H, Xu D, Sun H, Marangoni RG, et al.for 2 h at room temperature prior to incubation with primary rabbit antibodies against A20 (Abcam, ab92324; EPR2663, rabbit monoclonal, 1:200) or DREAM (Santa Cruz, sc166916, mouse monoclonal, 1:100) for overnight.

(10) YTHDF2 promotes temozolomide resistance in glioblastoma by activation of the Akt and NF‐κB signalling pathways via inhibiting EPHB3 and TNFAIP3Clinical & Translational ImmunologyMay 9, 2022Yu Chen, Yan‐Lan Wang, Kai Qiu, Yi‐Qiang Cao, Feng‐Jiang Zhang, Hai‐Biao Zhao, Xian‐Zhi Liuprimary antibodies including anti‐YTHDF2 (ab220163, Abcam), anti‐EPHB3 (ab133742, Abcam), anti‐TNFAIP3 (ab92324, Abcam), anti‐pAkt (Ser473, #3787, Cell Signaling technology, Bossdun, USA), anti‐Akt (#2938, Cell Signaling),

(11) A20 Attenuates the Fibrotic Response in the Trabecular MeshworkInternational Journal of Molecular SciencesFebruary 9, 2022Philip Mzyk, Emma G. Zalog, Colleen M. McDowellrabbit anti-fibronectin (AB1945, Millipore, Burlington, MA, USA) dilution 1:5000 and rabbit anti-A20 (ab92324, Abcam, Waltham, MA, USA) dilution 1:1000. The next day, membranes were incubated for 1 h at room temperature

(12) MicroRNA miR-27b-3p regulate microglial inflammation response and cell apoptosis by inhibiting A20 (TNF-α-induced protein 3)BioengineeredDecember 20, 2021Liping Li, Chao Qi, Yuanyuan Liu, Youliang Shen, Xia Zhao, Han Qin, Yi Zhang, Tengbo Yuincluded anti-IL-1β (ab2105, Abcam), anti-IL-6 (ab7737, Abcam), anti-TNF-α (ab66579, Abcam), Anti-TNFAIP3 (ab92324, Abcam) and anti-β-actin (ab8227, Abcam). Dual luciferase reporting system Briefly, 293 T cells were

(13) Expression of deubiquitinases in human gingiva and cultured human gingival fibroblastsBMC Oral HealthDecember 1, 2021Yong-Wei Fu, Hong-Zhi Xustimuli. Methods Antibodies and reagents Rabbit anti-CYLD (catalog #ab137524), rabbit anti-A20 (catalog #ab92324) and rabbit anti-OTULIN (catalog #ab151117) were purchased from Abcam (Cambridge, UK). Rabbit anti-p65

(14) A novel fatty acid-binding protein 5 and 7 inhibitor ameliorates oligodendrocyte injury in multiple sclerosis mouse modelsEBioMedicineOctober 1, 2021Cheng A, Jia W, Kawahata I, Fukunaga K.2314110 Iba-1,Fujifilm,016-20001,1:1000,AB_839506 4-HNE,JalCA,MHN-100P,1:1000,AB_1106813 TNF-α,Abcam,ab92324,1:500,AB_10561788 IL-1β,Abcam,ab9722,1:1000,AB_308765 MBP,Abcam,ab7349,1:500,AB_305869 FABP5,R&D Systems

(15) MK801 regulates the expression of key osteoarthritis factors in osteoarthritis synovial fibroblasts through complement C5Research in veterinary scienceMay 1, 2021Huang Z, Feng Y, Zhu X, Wang L, Lu W.company. Antibodies: Anti-C5 114 (ab17930), Anti-GAPDH (ab8245), Anti-C5b-9 (ab55811), Anti-TNFA (ab92324), 115 Anti-MMP13 (ab51072), Anti-Lamin B1 (ab16048)， Anti-NF-kB-p65 (ab16502) and 116 Anti-NFkB-p105/p50

(16) Potential of the tumor‑derived extracellular vesicles carrying the miR‑125b‑5p target TNFAIP3 in reducing the sensitivity of diffuse large B cell lymphoma to rituximabInternational Journal of OncologyApril 21, 2021Li Zhang, Shixia Zhou, Tiejun Zhou, Xiaoming Li, Junling Tangbuffer (Beyotime) for 1 h at room temperature and cultured with the primary antibodies TNFAIP3 (1:1,000, ab92324, Abcam) and CD20 (1:1,000, Abcam) overnight at 4°C. Subsequently, the membranes were cultured with the

(17) Identification of TNFAIP3 as relapse biomarker and potential therapeutic target for MOG antibody associated diseasesScientific reportsJuly 24, 2020Saxena S, Lokhande H, Gombolay G, Raheja R, Rooney T, Chitnis T.5% BSA (Sigma Aldrich, MO, USA) and blotted overnight at 4 °C with antibodies against anti-TNFAIP3 (ab92324, 1:1,000), anti- NFκβ p50 (ab32360, 1:2000) Abcam, MA, USA, anti-phospho NFκβ p65 (3033L, 1:2000), anti-

(18) A20 inhibits osteoclastogenesis via TRAF6-dependent autophagy in human periodontal ligament cells under hypoxiaCell proliferationMarch 1, 2020Yan K, Wu C, Ye Y, Li L, Wang X, He W, Ren S, Xu Y.against GAPDH (1:1000, Proteintech # 10494‐1‐AP), β‐actin (1:1000, Boster # BM0627), A20 (1:1000, Abcam # ab92324), RANKL (1:1000, Abcam # ab9957), OPG (1:1000, Abcam # ab73400), HIF‐1α (1:1000, CST # 36169), LC3B

(19) Downregulation of A20 Expression Increases the Immune Response and Apoptosis and Reduces Virus Production in Cells Infected by the Human Respiratory Syncytial VirusVaccinesFebruary 24, 2020Martín-Vicente M, González-Sanz R, Cuesta I, Monzón S, Resino S, Martínez I.previously described [31] using the following primary antibodies: anti-β-actin (Ab8224, Abcam), anti-A20 (Ab92324, Abcam) and anti-HRSV nucleoprotein (79N) [32]. 2.5. siRNA Silencing A549 cells were seeded 24 h before

(20) High A20 expression negatively impacts survival in patients with breast cancerPLOS ONEAugust 26, 2019Chang Ik Yoon et al.Seoul, Korea) [17]. For IHC, each TMA slide was stained with a rabbit monoclonal anti-A20 antibody (ab92324, 1:200; Abcam) and counter-stained with hematoxylin. After staining, A20 expression, assessed as cytoplasmic staining,

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Anti-TNFAIP3 antibody [EPR2663] from Abcam

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Anti-TNFAIP3 antibody [EPR2663] from Abcam

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